Anti-NOD2 Antibody, clone 2D9

Protocol tips

Upstream tips	Protocol tips	Downstream tips
	-Mouse (1:500)

Protocol tips
-Mouse (1:500)

Publication protocol

One volume of 2× loading buffer (60 mm Tris-HCl, pH 6.8, 2.5% SDS, 10% glycerol, 5% β-mercaptoethanol, and 0.03% bromphenol blue) was added to protein samples. After boiling for 5 min, SDS-PAGE was performed, and proteins were electrotransferred to PVDF membranes (Roche Applied Science). After probing with primary and secondary antibodies, membranes were finally analyzed by chemiluminescence with an ECL system (Amersham Biosciences).

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Manufacturer protocol

Download the product protocol from Merck Millipore for Anti-NOD2 Antibody, clone 2D9 below.

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