FAS Antibody (B-10): sc-8009

Protocol tips

Upstream tips	Protocol tips	Downstream tips
	-Mouse (1:1000) -Use clean forceps to gently handle the blot from the corner without creasing the membrane. Do not write on the blot with pen or marker, as the ink can fluoresce and cause background.

Protocol tips
-Mouse (1:1000) -Use clean forceps to gently handle the blot from the corner without creasing the membrane. Do not write on the blot with pen or marker, as the ink can fluoresce and cause background.

Publication protocol

Whole cell lysates were prepared using RIPA buffer (Sigma-Aldrich). After centrifugation at 14000 rpm for 15 min, supernatants were collected and analyzed for protein concentration using BCA Protein Assay Kit (ThermoFisher Scientific). Equal amounts of total proteins were resolved onto SDS-PAGE using 4–12% NuPAGE Bis-Tris gel (Life Technologies), transferred to PVDF membranes, incubated with specific antibodies, and visualized by ECL reagents. The immunoblots were subjected to densitometry analysis using the LAS-4000 Luminescent Image Analyzer (Fujifilm).

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Manufacturer protocol

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