ABC1 Antibody (AB.H10): sc-58219

Protocol tips

Upstream tips	Protocol tips	Downstream tips
	-Mouse (1:2000) -Use clean forceps to gently handle the blot from the corner without creasing the membrane. Do not write on the blot with pen or marker, as the ink can fluoresce and cause background.

Protocol tips
-Mouse (1:2000) -Use clean forceps to gently handle the blot from the corner without creasing the membrane. Do not write on the blot with pen or marker, as the ink can fluoresce and cause background.

Publication protocol

Western blot analysis were performed as described previously (5). The following primary antibodies were used: anti-ABCA1 (mouse; Santa Cruz Biotechnology; catalogue no. sc-58219; 1:2000), anti-CPT1A (rabbit; Proteintech; catalogue no. 15184-1-AP; 1:1000), anti-NPC1L1 (rabbit; Cell Signaling Technology; catalogue no. 5058; 1:500), anti-PPARα (rabbit; Santa Cruz Biotechnology; catalogue no. sc-9000; 1:500), anti-LXR (rabbit; Santa Cruz Biotechnology; catalogue no. sc-13068; 1:1000), anti-AEG-1 (chicken; in-house; 1:3000) and anti-GAPDH (mouse; Santa Cruz Biotechnology; catalogue no. sc-166545; 1:2500).

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Manufacturer protocol

Download the product protocol from Santa Cruz Biotechnology for ABC1 Antibody (AB.H10): sc-58219 below.

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