Human/Mouse/Rat SOX2 Antibody

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	-Mouse (1:2000)

Protocol tips
-Mouse (1:2000)

Publication protocol

Western blot analyses were performed as described previously (30). The primary antibodies used in the Western blot analyses include anti-BRD4 N (targeting BRD4 156–284; 1:40,000); anti-BRD4 C (targeting BRD4 1313–1362; 1:40,000); anti-NUT (targeting NUT 300–700; 1:20,000); anti-CDK9 (1:500; sc13130; Santa Cruz); anti-cyclin T1 (1:500; sc271575; Santa Cruz); anti-SOX2 (1:2,000; MAB2018; R&D); anti–c-MYC (1:2,000; 9402S; Cell Signaling Technology); anti-cytokeratin (1:4,000; M3515; Dako); anti-involucrin (1:2,000; I9018; Sigma); anti-GAPDH (1:4,000; G8140-01; US Biological); anti-Xpress (1:5,000; R910-25l; Invitrogen); and anti-HEXIM1 (1:2,000; 9064S; Cell Signaling Technology). HRP-linked anti-rabbit IgG (1:3,000; 7074S; Cell Signaling Technology) and HRP-linked anti-mouse IgG (1:3,000; 7076S; Cell Signaling Technology) were used as secondary antibodies. Western blots were developed using SuperSignal West Pico Chemiluminescent Substrate (Thermo Scientific), and images were captured using an Amersham Imager 600 (GE Healthcare).

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Manufacturer protocol

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