Cyclin D1 Recombinant Rabbit Monoclonal Antibody (SP4)

Protocol tips

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	-Rabbit (1:1000)

Protocol tips
-Rabbit (1:1000)

Publication protocol

Cells were prepared for WB analysis, as previously described.32 A list of the primary antibodies used is shown in Table 1. The membrane was then washed and incubated with the respective secondary antibodies conjugated with peroxidase (Amersham Pharmacia Biotech, Buckinghamshire, UK) for 1 hour, and the immune complex was visualized with the electrochemiluminescence system (Santa Cruz Biotechnology, Santa Cruz, CA).

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Manufacturer protocol

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