Stat3 Antibody (F-2): sc-8019

Western blotting STAT3

Experiment
Western blotting STAT3
Product
Stat3 Antibody (F-2): sc-8019 from Santa Cruz Biotechnology
Manufacturer
Santa Cruz Biotechnology

Protocol tips

Protocol tips
-Mouse (1:200)
-Use clean forceps to gently handle the blot from the corner without creasing the membrane. Do not write on the blot with pen or marker, as the ink
can fluoresce and cause background.

Publication protocol

Liver allograft samples were rapidly ground in liquid nitrogen, and the cultured HSCs were harvested. The tissue powder or the isolated HSC cells were reconstituted in ice-cold RIPA buffer with protease and phosphatase inhibitors. Supernatants were recovered after centrifugation and assayed for protein content using the BCA method (Beyotime Biotechnology, P0010). Protein samples of 50 mg per lane were separated by 8% SDS-polyacrylamide gel electrophoresis (SDS-PAGE) and transferred to polyvinylidene-difluoride (PVDF) membranes. The membranes were incubated in 5% skimmed milk for 2 h at 37°C and overnight at 4°C with primary antibodies (Santa Cruz) (rat anti-CXCR3, 1 : 200 dilution; EGFR, 1 : 200 dilution; JAK2, 1 : 200 dilution; STAT3, 1 : 200 dilution; Collagen IV, 1 : 200 dilution). GAPDH protein expression was used as a loading control. The membranes were exposed to the negative films to develop target bands after incubation with enhanced chemiluminescence (Santa Cruz, USA). The intensities of bands were quantitated by LabWorks 4.5 software (UVP, USA).

Full paper   Login or join for free to view the full paper.

Reviews

Stat3 Antibody (F-2): sc-8019 from Santa Cruz Biotechnology has not yet been reviewed for this experiment

We'd love it if you would be the first to write a review!

Discussion

Start your discussion

Share your thoughts or question with experts in your field

Start a discussion

Papers

Check out relevant papers found by Labettor's AI that are relevant for performing Western blotting STAT3 using Stat3 Antibody (F-2): sc-8019 from Santa Cruz Biotechnology.

View full paper   Login or join for free to view the full paper.

Manufacturer protocol

Download the product protocol from Santa Cruz Biotechnology for Stat3 Antibody (F-2): sc-8019 below.

Download PDF Download manufacturer protocol

Videos

Check out videos that might be relevant for performing Western blotting STAT3 using Stat3 Antibody (F-2): sc-8019 from Santa Cruz Biotechnology. Please note that these videos are representative and steps or experiment specific processes must be kept in mind to expect desired results.

We haven't found any additional videos for this experiment / product combination yet.

Outsource your experiment

Fill out your contact details and receive price quotes in your Inbox

  Outsource experiment
Become shareholder Discussions About us Contact Privacy Terms