SOX9 monoclonal antibody (M04), clone 3F11

Protocol tips

Upstream tips	Protocol tips	Downstream tips
-Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing. -Reagent must be freshly prepared freshly each time before application.	-Mouse (1:500) -Please keep the membranes at -20°C no longer than a week if it will not be used immediately

Upstream tips
-Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing. -Reagent must be freshly prepared freshly each time before application.
Protocol tips
-Mouse (1:500) -Please keep the membranes at -20°C no longer than a week if it will not be used immediately

Publication protocol

Cells were lysed with RIPA containing with 1 mM PMSF. Then used the BCA protein assay kit was used to measure protein concentrations. The 5 μl marker and 25 μg proteins were separated by 10% SDS-PAGE gels and transferred to PVDF membranes. Membranes were blocked 2 h at RT with 5% skim milk (diluted in TBS), and then incubated with primary antibodies at 4 °C shaking for overnight. Followed by second antibodies anti-rabbit /mouse were blocked 2 h at RT. According to the ECL kit (Enhanced chemiluminescence system kit) protocol, detection was performed.

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Manufacturer protocol

Download the product protocol from Abnova for SOX9 monoclonal antibody (M04), clone 3F11 below.

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