Monoclonal Anti-γ-Tubulin antibody produced in mouse

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	-Mouse (1:1000)

Protocol tips
-Mouse (1:1000)

Publication protocol

Antibodies used for immunoblot analysis were as follows: ms α‐FBXO7 (1:50 dilution; Santa Cruz, sc‐271763), ms α‐γ‐tubulin (1:1,000 dilution; Sigma‐Aldrich, T6557), rb α‐PSMA2 (1:1,000 dilution; Cell Signaling, 2455), rb α‐PSMA2 (in vitro ubiquitination assay, 1:500 dilution; LSBio, L‐C138512), gt α‐PI31 (1:500 dilution; Sigma‐Aldrich, SAB2500788), ms α‐Myc (1:500 dilution; Santa Cruz, sc‐40), ms α‐Flag (1:1,000 dilution; Sigma‐Aldrich, F1804), ms α‐pan 14‐3‐3 (1:5,000 dilution; Santa Cruz, sc‐1657), ms α‐α‐synuclein (1:1,000 dilution; Cell Signaling, 4179), ms α‐β‐galactosidase (1:500 dilution; Santa Cruz, sc‐65670), ms α‐SP1 (1:500 dilution; Santa Cruz, sc‐14027), ms α‐Rpt6 (1:500 dilution; Enzo Lifescience, p45‐110), ms α‐K63‐linked ubiquitin (1:200 dilution; Millipore, 05‐1308), ms α‐K48‐linked ubiquitin (1:200 dilution; Millipore, 05‐1307), rb α‐ubiquitin (1:500 dilution; DAKO, Z0458), ms α‐ubiquitin P4D1 (1:500 dilution; Santa Cruz, sc‐8017), ms α‐GFP (1:500 dilution; Santa Cruz, sc‐9996), ms α‐HA (1:500 dilution; Santa Cruz, sc‐805), ms α‐cullin‐1 (1:500 dilution; Santa Cruz, sc‐17775), ms α‐GAPDH (1:500 dilution; mAbcam 9484).

HRP‐coupled secondary antibodies were goat α‐rabbit (1:1,000; Dianova, 115‐035‐144), goat α‐mouse (1:1,000; Dianova, 11‐035‐146), donkey α‐goat (1:1,000 Santa Cruz, sc‐2020).

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Manufacturer protocol

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