PARP-1 Antibody (F-2): sc-8007

Protocol tips

Upstream tips	Protocol tips	Downstream tips
	-Mouse (1:1000) -Use clean forceps to gently handle the blot from the corner without creasing the membrane. Do not write on the blot with pen or marker, as the ink can fluoresce and cause background.

Protocol tips
-Mouse (1:1000) -Use clean forceps to gently handle the blot from the corner without creasing the membrane. Do not write on the blot with pen or marker, as the ink can fluoresce and cause background.

Publication protocol

Total cell lysates or the immunoprecipitated proteins were finally analysed by SDS-PAGE. Antibodies against PAR (ab14459, 1:500 for WB), γH2AX (phosphor S139) (ab2893, 1:200 for IF and 1:1000 for WB), Histone H3 (ab10799, 1:1000 for WB), Histone H3 (acetyl k9) (ab10812, 1:500 for WB) from Abcam; ATM (NB100-309, 1:1000 for WB) from Novus Biologicals; PARP-1 (sc-8007, IP and 1:1000 for WB) from Santa Cruz Biotechnology, SMARCA5 (A2000, IP and 1:1500 for WB) from abclonal; GCN5 (3305, IP and 1:1000 for WB), p53 (2524, 1:1000 for WB) from CST; acety-Histone H3 (06-599, IP and 1:20,000 for WB) from Millipore. DNA Damage Antibody Sampler Kit (9947) was purchased from CST. The mouse monoclonal anti-Tudor-SN antibody was generated against the SN4 domain (amino acids 507–674) of Tudor-SN at the Institute of Medical Technology, University of Tampere, Finland.

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Manufacturer protocol

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