Anti-Cleaved PARP1 antibody [4B5BD2] (ab110315)

Protocol tips

Upstream tips	Protocol tips	Downstream tips
-These buffers may be stored at 4°C for several weeks or aliquoted and stored at -20°C for up to a year.	-Mouse (1:1000) -To reduce and denature your samples, boil each cell lysate in sample buffer at 100°C for 5 min. Lysates can be aliquoted and stored at -20°C for future use.

Upstream tips
-These buffers may be stored at 4°C for several weeks or aliquoted and stored at -20°C for up to a year.
Protocol tips
-Mouse (1:1000) -To reduce and denature your samples, boil each cell lysate in sample buffer at 100°C for 5 min. Lysates can be aliquoted and stored at -20°C for future use.

Publication protocol

Immunoblot analysis was performed using standard protocols using horseradish peroxidase (HRP)-conjugated secondary antibodies (Biozol). Proteins were detected using WesternBright chemiluminescent solution (Advansta) or SuperSignal West Femto reagent (Thermo Fisher Scientific). Images were acquired on a ChemiDoc MP Imaging System (Bio-Rad). Band intensities were quantified with Fiji software.

Full paper   Login or join for free to view the full paper.

Reviews

Discussion

Papers

Check out relevant papers found by Labettor's AI that are relevant for performing Western blotting PARP using Anti-Cleaved PARP1 antibody [4B5BD2] (ab110315) from Abcam.

Manufacturer protocol

Download the product protocol from Abcam for Anti-Cleaved PARP1 antibody [4B5BD2] (ab110315) below.

Download manufacturer protocol

Videos

Check out videos that might be relevant for performing Western blotting PARP using Anti-Cleaved PARP1 antibody [4B5BD2] (ab110315) from Abcam. Please note that these videos are representative and steps or experiment specific processes must be kept in mind to expect desired results.