Gibco™DMEM/F-12

Protocol tips

Upstream tips	Protocol tips	Downstream tips
A total of 50,000 single ESCs resuspended in 10 μl N2B27 were embedded in 150 μl Matrigel and distributed equally over five 3.5 cm2 glass bottom dishes (MatTek)	the embedded cells were cultured in N2B27 consisted of DMEM/F12 + N2 medium 25 mL, Neurobasal + B27 medium 25 mL, β-Mercaptoethanol (100 mM) 50 µL

Upstream tips
A total of 50,000 single ESCs resuspended in 10 μl N2B27 were embedded in 150 μl Matrigel and distributed equally over five 3.5 cm2 glass bottom dishes (MatTek)
Protocol tips
the embedded cells were cultured in N2B27 consisted of DMEM/F12 + N2 medium 25 mL, Neurobasal + B27 medium 25 mL, β-Mercaptoethanol (100 mM) 50 µL

Publication protocol

the embedded cells were cultured in N2B27 consisted of DMEM/F12 + N2 medium 25 mL, Neurobasal + B27 medium 25 mL, β-Mercaptoethanol (100 mM) 50 µL

Full paper   Login or join for free to view the full paper.

Reviews

Discussion

Papers

Check out relevant papers found by Labettor's AI that are relevant for performing Stem cell Differentiation media mESCs differentiation into Neuroepithelial Cyst using Gibco™DMEM/F-12 from Thermo Fisher Scientific.

Manufacturer protocol

Download the product protocol from Thermo Fisher Scientific for Gibco™DMEM/F-12 below.

Download manufacturer protocol

Videos

Check out videos that might be relevant for performing Stem cell Differentiation media mESCs differentiation into Neuroepithelial Cyst using Gibco™DMEM/F-12 from Thermo Fisher Scientific. Please note that these videos are representative and steps or experiment specific processes must be kept in mind to expect desired results.