Gibco™IMDM

Protocol tips

Upstream tips	Protocol tips	Downstream tips
human pluripotent stem cells were detached from the feeder layer by collagenase IV, dispersed into small clumps	Iscove’s modified Dulbecco’s differentiation medium supplemented with 20% fetal calf serum, 1 mM l-glutamine, 0.1 mM beta-mercaptoethanol and 1% nonessential amino acid stock in ultra-low attachment plates (Corning) for 7 days.

Upstream tips
human pluripotent stem cells were detached from the feeder layer by collagenase IV, dispersed into small clumps
Protocol tips
Iscove’s modified Dulbecco’s differentiation medium supplemented with 20% fetal calf serum, 1 mM l-glutamine, 0.1 mM beta-mercaptoethanol and 1% nonessential amino acid stock in ultra-low attachment plates (Corning) for 7 days.

Publication protocol

Iscove’s modified Dulbecco’s differentiation medium supplemented with 20% fetal calf serum, 1 mM l-glutamine, 0.1 mM beta-mercaptoethanol and 1% nonessential amino acid stock in ultra-low attachment plates (Corning) for 7 days.

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Discussion

Papers

Check out relevant papers found by Labettor's AI that are relevant for performing Stem cell Differentiation media hiPSCs or hESCs differentiation to Embryoid body (EB) using Gibco™IMDM from Thermo Fisher Scientific.

Manufacturer protocol

Download the product protocol from Thermo Fisher Scientific for Gibco™IMDM below.

Download manufacturer protocol

Videos

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