Gibco™Glasgow's MEM (GMEM)

Protocol tips

Upstream tips	Protocol tips	Downstream tips
	differentiation medium (G-MEM (Gibco #11710-035), supplemented with 10% KnockOut™ Serum Replacement (Gibco #10828-028, Lot 1667387), 1% non-essential amino acids (GE Healthcare), 0.1 mM β-mercaptoethanol (Sigma Aldrich), 200 U/ml penicillin (Sigma Aldrich), 200 μg/ml streptomycin (Sigma Aldrich)) per 1/96 well (Nunclon Sphera® Low Adhesion Plate 96-U, Thermo Scientific #174925).	On day six, aggregates were transferred to 90 mm plates with low adhesion surface (Nunclon Sphera® 90 mm, Thermo Scientific #174945) and subsequently cultured in N2 medium (49% DMEM low glucose (1 g/l, GE Healthcare #E15-005), 49% HAM's F12 (GE Healthcare # E15-016), 1 × N2 supplement (100 ×, Gibco #17502-048 Lot 1737260) and 200 U/ml penicillin (Sigma Aldrich), 200 μg/ml streptomycin (Sigma Aldrich)).

Protocol tips
differentiation medium (G-MEM (Gibco #11710-035), supplemented with 10% KnockOut™ Serum Replacement (Gibco #10828-028, Lot 1667387), 1% non-essential amino acids (GE Healthcare), 0.1 mM β-mercaptoethanol (Sigma Aldrich), 200 U/ml penicillin (Sigma Aldrich), 200 μg/ml streptomycin (Sigma Aldrich)) per 1/96 well (Nunclon Sphera® Low Adhesion Plate 96-U, Thermo Scientific #174925).
Downstream tips
On day six, aggregates were transferred to 90 mm plates with low adhesion surface (Nunclon Sphera® 90 mm, Thermo Scientific #174945) and subsequently cultured in N2 medium (49% DMEM low glucose (1 g/l, GE Healthcare #E15-005), 49% HAM's F12 (GE Healthcare # E15-016), 1 × N2 supplement (100 ×, Gibco #17502-048 Lot 1737260) and 200 U/ml penicillin (Sigma Aldrich), 200 μg/ml streptomycin (Sigma Aldrich)).

Publication protocol

differentiation medium (G-MEM (Gibco #11710-035), supplemented with 10% KnockOut™ Serum Replacement (Gibco #10828-028, Lot 1667387), 1% non-essential amino acids (GE Healthcare), 0.1 mM β-mercaptoethanol (Sigma Aldrich), 200 U/ml penicillin (Sigma Aldrich), 200 μg/ml streptomycin (Sigma Aldrich)) per 1/96 well (Nunclon Sphera® Low Adhesion Plate 96-U, Thermo Scientific #174925).

Full paper   Login or join for free to view the full paper.

Reviews

Discussion

Papers

Check out relevant papers found by Labettor's AI that are relevant for performing Stem cell Differentiation media mPSCs/mESCs differentiation to Embryoid body (EB) using Gibco™Glasgow's MEM (GMEM) from Thermo Fisher Scientific.

Manufacturer protocol

Download the product protocol from Thermo Fisher Scientific for Gibco™Glasgow's MEM (GMEM) below.

Download manufacturer protocol

Videos

Check out videos that might be relevant for performing Stem cell Differentiation media mPSCs/mESCs differentiation to Embryoid body (EB) using Gibco™Glasgow's MEM (GMEM) from Thermo Fisher Scientific. Please note that these videos are representative and steps or experiment specific processes must be kept in mind to expect desired results.