Adipogenic Induction Medium

Stem cell Differentiation media mPericytes differentiation into adipogenic cells

Experiment
Stem cell Differentiation media mPericytes differentiation into adipogenic cells
Product
Adipogenic Induction Medium from Lonza
Manufacturer
Lonza

Protocol tips

Upstream tips
cultured with Mesenchymal Stem Cell Basal Medium (MSCBM, Lonza, Basel, Switzerland, #PT-3238) until the cells became 100% confluent.
Protocol tips
adipogenic induction medium (Lonza, #PT-3102B) supplemented with h-insulin, L-glutamine, mesenchymal cell growth supplement (MCGS), dexamethasone, indomethacin, isobutylmethylxanthine (IBMX), and GA-1000 (Lonza, #PT-4135) for 4 days. Then, the cells were cultured in adipogenic maintenance medium (Lonza, #PT-3102A) supplemented with h-insulin, L-glutamine, MCGS, and GA-1000 (Lonza, #PT-4122)

Publication protocol

adipogenic induction medium (Lonza, #PT-3102B) supplemented with h-insulin, L-glutamine, mesenchymal cell growth supplement (MCGS), dexamethasone, indomethacin, isobutylmethylxanthine (IBMX), and GA-1000 (Lonza, #PT-4135) for 4 days. Then, the cells were cultured in adipogenic maintenance medium (Lonza, #PT-3102A) supplemented with h-insulin, L-glutamine, MCGS, and GA-1000 (Lonza, #PT-4122)

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Manufacturer protocol

Download the product protocol from Lonza for Adipogenic Induction Medium below.

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