MegaCell™ Dulbecco’s Modified Eagle’s Medium

Protocol tips

Upstream tips	Protocol tips	Downstream tips
	DMEM)-mega-cell, supplemented with 10% FBS, 2 mM glutamine, 100 U/ml penicillin-streptomycin amphotericin, 1% non-essential amino acids (Lonza, Switzerland), 0.1 mM β-mercaptoethanol (Serva, Germany), 5 ng/ml basic fibroblast growth factor (Gibco, USA) and 10 µM 5-Aza-2'-deoxycytidine (5-Aza) (Abcam, UK).

Protocol tips
DMEM)-mega-cell, supplemented with 10% FBS, 2 mM glutamine, 100 U/ml penicillin-streptomycin amphotericin, 1% non-essential amino acids (Lonza, Switzerland), 0.1 mM β-mercaptoethanol (Serva, Germany), 5 ng/ml basic fibroblast growth factor (Gibco, USA) and 10 µM 5-Aza-2'-deoxycytidine (5-Aza) (Abcam, UK).

Publication protocol

DMEM)-mega-cell, supplemented with 10% FBS, 2 mM glutamine, 100 U/ml penicillin-streptomycin amphotericin, 1% non-essential amino acids (Lonza, Switzerland), 0.1 mM β-mercaptoethanol (Serva, Germany), 5 ng/ml basic fibroblast growth factor (Gibco, USA) and 10 µM 5-Aza-2'-deoxycytidine (5-Aza) (Abcam, UK).

Full paper   Login or join for free to view the full paper.

Reviews

Discussion

Papers

Check out relevant papers found by Labettor's AI that are relevant for performing Stem cell Differentiation media hPericytes differentiation into myogenic cells using MegaCell™ Dulbecco’s Modified Eagle’s Medium from Sigma-Aldrich.

Manufacturer protocol

Download the product protocol from Sigma-Aldrich for MegaCell™ Dulbecco’s Modified Eagle’s Medium below.

Download manufacturer protocol

Videos

Check out videos that might be relevant for performing Stem cell Differentiation media hPericytes differentiation into myogenic cells using MegaCell™ Dulbecco’s Modified Eagle’s Medium from Sigma-Aldrich. Please note that these videos are representative and steps or experiment specific processes must be kept in mind to expect desired results.