DMEM/F-12 with 15 mM HEPES

Protocol tips

Upstream tips	Protocol tips	Downstream tips
cultured on 0.1% gelatin (Sigma) coated plates on human foreskin fibroblasts (ATCC CRL-2429)	DMEM F12 (Stem Cell Technologies), 20% Serum Replacement, Knockout SR (Gibco), 2 mM Glutamax (Gibco), 1% Non-essential Aminoacids (Gibco), 48,5 U/ml Penicillin-Streptomycin (Gibco), 0.1 mM 2-mercaptoethanol (Gibco), 4 ng/ml rhFGF (R&D Systems

Upstream tips
cultured on 0.1% gelatin (Sigma) coated plates on human foreskin fibroblasts (ATCC CRL-2429)
Protocol tips
DMEM F12 (Stem Cell Technologies), 20% Serum Replacement, Knockout SR (Gibco), 2 mM Glutamax (Gibco), 1% Non-essential Aminoacids (Gibco), 48,5 U/ml Penicillin-Streptomycin (Gibco), 0.1 mM 2-mercaptoethanol (Gibco), 4 ng/ml rhFGF (R&D Systems

Publication protocol

DMEM F12 (Stem Cell Technologies), 20% Serum Replacement, Knockout SR (Gibco), 2 mM Glutamax (Gibco), 1% Non-essential Aminoacids (Gibco), 48,5 U/ml Penicillin-Streptomycin (Gibco), 0.1 mM 2-mercaptoethanol (Gibco), 4 ng/ml rhFGF (R&D Systems

Full paper   Login or join for free to view the full paper.

Reviews

Discussion

Papers

Check out relevant papers found by Labettor's AI that are relevant for performing Stem cell culture media hESC lines H9, H1 using DMEM/F-12 with 15 mM HEPES from STEMCELL technologies.

Manufacturer protocol

Download the product protocol from STEMCELL technologies for DMEM/F-12 with 15 mM HEPES below.

Download manufacturer protocol

Videos

Check out videos that might be relevant for performing Stem cell culture media hESC lines H9, H1 using DMEM/F-12 with 15 mM HEPES from STEMCELL technologies. Please note that these videos are representative and steps or experiment specific processes must be kept in mind to expect desired results.