Corning® 500 mL SF Medium, [+] L-glutamine and 1 g/L BSA

Protocol tips

Upstream tips	Protocol tips	Downstream tips
density of 50,000 cells/ml	serum-free DMEM/F12 medium (Cellgro, Virginia, USA) composed of 10 ng/ml basic fibroblast growth factor (bFGF, Peprotech, Rocky Hill, NJ, USA) and 20 ng/ml epidermal growth factor (EGF, Peprotech), 1 mg/ML insulin (Sigma–Aldrich, St Louis, MO, USA), and 1 × B27 supplement (Life Technologies,Carlsbad, CA, USA) on the ultralow attachplates (Corning, Steuben County, NY, USA)

Upstream tips
density of 50,000 cells/ml
Protocol tips
serum-free DMEM/F12 medium (Cellgro, Virginia, USA) composed of 10 ng/ml basic fibroblast growth factor (bFGF, Peprotech, Rocky Hill, NJ, USA) and 20 ng/ml epidermal growth factor (EGF, Peprotech), 1 mg/ML insulin (Sigma–Aldrich, St Louis, MO, USA), and 1 × B27 supplement (Life Technologies,Carlsbad, CA, USA) on the ultralow attachplates (Corning, Steuben County, NY, USA)

Publication protocol

serum-free DMEM/F12 medium (Cellgro, Virginia, USA) composed of 10 ng/ml basic fibroblast growth factor (bFGF, Peprotech, Rocky Hill, NJ, USA) and 20 ng/ml epidermal growth factor (EGF, Peprotech), 1 mg/ML insulin (Sigma–Aldrich, St Louis, MO, USA), and 1 × B27 supplement (Life Technologies,Carlsbad, CA, USA) on the ultralow attachplates (Corning, Steuben County, NY, USA)

Full paper   Login or join for free to view the full paper.

Reviews

Discussion

Papers

Check out relevant papers found by Labettor's AI that are relevant for performing Stem cell culture media Ovarian cancer stem cells (Caov3, 3AO, SKOV3) using Corning® 500 mL SF Medium, [+] L-glutamine and 1 g/L BSA from Corning.

Manufacturer protocol

Download the product protocol from Corning for Corning® 500 mL SF Medium, [+] L-glutamine and 1 g/L BSA below.

Download manufacturer protocol

Videos

Check out videos that might be relevant for performing Stem cell culture media Ovarian cancer stem cells (Caov3, 3AO, SKOV3) using Corning® 500 mL SF Medium, [+] L-glutamine and 1 g/L BSA from Corning. Please note that these videos are representative and steps or experiment specific processes must be kept in mind to expect desired results.