ON-TARGETplus Human PIK3CA (5290) siRNA - SMARTpool

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	A498 cell lines were acquired from the American Type Culture Collection (Manassas, VA, USA). Cells undergo annual STR analysis for genetic confirmation. All cell lines were cultured in Dulbecco’s Modified Eagle Medium (DMEM, Gibco/Life Technologies, Carlsbad, CA, USA) supplemented with 10% FBS (Gemini Bio-Products, West Sacramento, CA, USA), non-essential amino acids, L-glutamine, penicillin, and streptomycin. All cultures were maintained at 37° C in 5% CO2. Cells were transfected with human SMART pool ON-TARGETplus PIK3CA (L-003018-00-0005), and ON-TARGETplus Non-targeting pool (D-001810-10-05) siRNAs siRNAs using DharmaFECT 1 transfection reagent (T-2-001-02) (GE Dharmacon) according to the manufacturer’s protocol. Transfections were performed in serum-reduced, antibiotic-free DharmaFECT Cell Culture Reagent (DCCR) (B-004500-100) (GE Dharmacon).

Protocol tips

A498 cell lines were acquired from the American Type Culture Collection (Manassas, VA, USA). Cells undergo annual STR analysis for genetic confirmation. All cell lines were cultured in Dulbecco’s Modified Eagle Medium (DMEM, Gibco/Life Technologies, Carlsbad, CA, USA) supplemented with 10% FBS (Gemini Bio-Products, West Sacramento, CA, USA), non-essential amino acids, L-glutamine, penicillin, and streptomycin. All cultures were maintained at 37° C in 5% CO2. Cells were transfected with human SMART pool ON-TARGETplus PIK3CA (L-003018-00-0005), and ON-TARGETplus Non-targeting pool (D-001810-10-05) siRNAs siRNAs using DharmaFECT 1 transfection reagent (T-2-001-02) (GE Dharmacon) according to the manufacturer’s protocol. Transfections were performed in serum-reduced, antibiotic-free DharmaFECT Cell Culture Reagent (DCCR) (B-004500-100) (GE Dharmacon).

Publication protocol

A498 cell lines were acquired from the American Type Culture Collection (Manassas, VA, USA). Cells undergo annual STR analysis for genetic confirmation. All cell lines were cultured in Dulbecco’s Modified Eagle Medium (DMEM, Gibco/Life Technologies, Carlsbad, CA, USA) supplemented with 10% FBS (Gemini Bio-Products, West Sacramento, CA, USA), non-essential amino acids, L-glutamine, penicillin, and streptomycin. All cultures were maintained at 37° C in 5% CO2. Cells were transfected with human SMART pool ON-TARGETplus PIK3CA (L-003018-00-0005), and ON-TARGETplus Non-targeting pool (D-001810-10-05) siRNAs siRNAs using DharmaFECT 1 transfection reagent (T-2-001-02) (GE Dharmacon) according to the manufacturer’s protocol. Transfections were performed in serum-reduced, antibiotic-free DharmaFECT Cell Culture Reagent (DCCR) (B-004500-100) (GE Dharmacon).

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