Silencer® Select_ MYD88

siRNA / miRNA gene silencing Human - A375 MYD88

Experiment
siRNA / miRNA gene silencing Human - A375 MYD88
Product
Silencer® Select_ MYD88 from Thermo Fisher Scientific
Manufacturer
Thermo Fisher Scientific

Protocol tips

Protocol tips
The melanoma cell lines A375 (ATCC® CRL-1619; VA, USA) were purchased from American Type Culture Collection (ATCC). All cell lines had been tested for mycoplasma. A375 cells were cultured in DMEM (Gibco, Thermo Fisher, MA, USA), supplemented with 10% fetal bovine serum (FBS)/1% penicillin-streptomycin (Gibco). The MYD88 siRNA (s9138) and negative control siRNA (4390843) were bought from ThermoFisher. A375 cells were transfected with siRNAs using Lipofectamine RNAiMAX Reagent (ThermoFisher).

Publication protocol

The melanoma cell lines A375 (ATCC® CRL-1619; VA, USA) were purchased from American Type Culture Collection (ATCC). All cell lines had been tested for mycoplasma. A375 cells were cultured in DMEM (Gibco, Thermo Fisher, MA, USA), supplemented with 10% fetal bovine serum (FBS)/1% penicillin-streptomycin (Gibco). The MYD88 siRNA (s9138) and negative control siRNA (4390843) were bought from ThermoFisher. A375 cells were transfected with siRNAs using Lipofectamine RNAiMAX Reagent (ThermoFisher).

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Manufacturer protocol

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