Stealth siRNA_SPI1

siRNA / miRNA gene silencing Human - LAD2 PU.1/SPI1

Experiment
siRNA / miRNA gene silencing Human - LAD2 PU.1/SPI1
Product
Stealth siRNA_SPI1 from Thermo Fisher Scientific
Manufacturer
Thermo Fisher Scientific

Protocol tips

Protocol tips
PU.1 siRNA (Stealth Select RNAi, HSS144058, HSS144060, and HSS186060), GATA1 siRNA (HSS142150, HSS142151, and HSS142152), GATA2 siRNA (HSS104003, HSS104004, HSS178122), c-kit siRNA (HSS105821), and control siRNA (Stealth RNAi Negative Universal Control Lo GC, Med GC, and Hi GC [12935-200, 300, and 400]) were purchased from Invitrogen (Carlsbad, CA). Transfection of siRNA was performed, as described previously (14). Briefly, 10 (or 1) ml 20 mmol/L siRNA was introduced into 2 3 106 (or 2 3 105) LAD2 cells or primary mast cells with a Neon 100 ml kit (or a Neon 10 ml kit) using a Neon transfection system (Invitrogen) set at Program 16 (for LAD2) or 5 (for primary mast cells).

Publication protocol

PU.1 siRNA (Stealth Select RNAi, HSS144058, HSS144060, and HSS186060), GATA1 siRNA (HSS142150, HSS142151, and HSS142152), GATA2 siRNA (HSS104003, HSS104004, HSS178122), c-kit siRNA (HSS105821), and control siRNA (Stealth RNAi Negative Universal Control Lo GC, Med GC, and Hi GC [12935-200, 300, and 400]) were purchased from Invitrogen (Carlsbad, CA). Transfection of siRNA was performed, as described previously (14). Briefly, 10 (or 1) ml 20 mmol/L siRNA was introduced into 2 3 106 (or 2 3 105) LAD2 cells or primary mast cells with a Neon 100 ml kit (or a Neon 10 ml kit) using a Neon transfection system (Invitrogen) set at Program 16 (for LAD2) or 5 (for primary mast cells).

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Papers

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Manufacturer protocol

Download the product protocol from Thermo Fisher Scientific for Stealth siRNA_SPI1 below.

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