ON-TARGETplus Human NOS2 (4843) siRNA - SMARTpool

siRNA / miRNA gene silencing Human - Caco-2 iNOS/NOS2

Experiment
siRNA / miRNA gene silencing Human - Caco-2 iNOS/NOS2
Product
ON-TARGETplus Human NOS2 (4843) siRNA - SMARTpool from Horizon Discovery Ltd.
Manufacturer
Horizon Discovery Ltd.

Protocol tips

Protocol tips
"Caco-2 cells were treated with small interfering RNA (siRNA)using a modification of our previously published methods (Forsyth
et al., 2010). Briefly, 105 cells are combined with 11 picomoles of siRNA in 50 ll Lipofectamine (Invitrogen) and 50 ul Optimem (Invitrogen), mixed by gently shaking and then plated on Type 1 collagencoated Corning 12 mm PET culture inserts (#3460) with addition of another 400 ul (upper well) and 1 ml (lower well) Optimem. Inserts are used for permeability studies 72 to 96 hours after plating, when
the cells are confluent. PAK1 and iNOS siRNA were On Target
Smartpool from Dharmacon (Lafayette, CO), whereas control
(nontargeting) siRNA was from Santa Cruz Biotechnology (Santa
Cruz, CA)"

Publication protocol

Caco-2 cells were treated with small interfering RNA (siRNA)using a modification of our previously published methods (Forsyth
et al., 2010). Briefly, 105 cells are combined with 11 picomoles of siRNA in 50 ll Lipofectamine (Invitrogen) and 50 ul Optimem (Invitrogen), mixed by gently shaking and then plated on Type 1 collagencoated Corning 12 mm PET culture inserts (#3460) with addition of another 400 ul (upper well) and 1 ml (lower well) Optimem. Inserts are used for permeability studies 72 to 96 hours after plating, when
the cells are confluent. PAK1 and iNOS siRNA were On Target
Smartpool from Dharmacon (Lafayette, CO), whereas control
(nontargeting) siRNA was from Santa Cruz Biotechnology (Santa
Cruz, CA)

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Papers

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Manufacturer protocol

Download the product protocol from Horizon Discovery Ltd. for ON-TARGETplus Human NOS2 (4843) siRNA - SMARTpool below.

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