Accell Mouse Nrep (27528) siRNA - SMARTpool

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	3T3-L1 cells (CL-173™, ATCC) were cultured in Dulbecco's Modified Eagle Media high medium with 10% fetal calf serum (ATCC) and penstrep and used for the adipogenesis studies as described earlier [15]. Self delivering P311 siRNA, Accell Mouse Nrep (27528) siRNA - SMART pool (GE Healthcare Dharmacon, Inc., Lafayette, CO), was used for knockdown studies. A pool of four siRNA (75 mM) were delivered using 1X siRNA delivery medium. Non-targeting (NT-) siRNA and siRNA-green were used for experimental control and transfection efficiency studies, respectively.

Protocol tips

3T3-L1 cells (CL-173™, ATCC) were cultured in Dulbecco's Modified Eagle Media high medium with 10% fetal calf serum (ATCC) and penstrep and used for the adipogenesis studies as described earlier [15]. Self delivering P311 siRNA, Accell Mouse Nrep (27528) siRNA - SMART pool (GE Healthcare Dharmacon, Inc., Lafayette, CO), was used for knockdown studies. A pool of four siRNA (75 mM) were delivered using 1X siRNA delivery medium. Non-targeting (NT-) siRNA and siRNA-green were used for experimental control and transfection efficiency studies, respectively.

Publication protocol

3T3-L1 cells (CL-173™, ATCC) were cultured in Dulbecco's Modified Eagle Media high medium with 10% fetal calf serum (ATCC) and penstrep and used for the adipogenesis studies as described earlier [15]. Self delivering P311 siRNA, Accell Mouse Nrep (27528) siRNA - SMART pool (GE Healthcare Dharmacon, Inc., Lafayette, CO), was used for knockdown studies. A pool of four siRNA (75 mM) were delivered using 1X siRNA delivery medium. Non-targeting (NT-) siRNA and siRNA-green were used for experimental control and transfection efficiency studies, respectively.

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