CD326 (EpCAM) Monoclonal Antibody (G8.8), eFluor 450, eBioscience™

Protocol tips

Upstream tips	Protocol tips	Downstream tips
	Five million cells per animal were blocked with 1 µg Fc-block/tube and stained or corresponding isotype antibodies for 20 min at 4°C.	For FACS analysis a FACSCanto II (BD Biosciences) was used. Results were visualized by the FlowJo package (Tree Star Inc., Ashland, OR, USA).

Protocol tips
Five million cells per animal were blocked with 1 µg Fc-block/tube and stained or corresponding isotype antibodies for 20 min at 4°C.
Downstream tips
For FACS analysis a FACSCanto II (BD Biosciences) was used. Results were visualized by the FlowJo package (Tree Star Inc., Ashland, OR, USA).

Publication protocol

For FACS analysis a FACSCanto II (BD Biosciences) was used. Five million cells per animal were blocked with 1 µg Fc-block/tube and stained with anti-mouse CD31 antibody (PE-conjugated, clone 390; Biolegend, 1 µg/ml), anti-mouse CD34 antibody (FITC-conjugated, clone RAM34; eBioscience, 10 µg/ml), anti-mouse CD45 antibody (PerCP/Cy5.5-conjugated, clone 30-F11; Biolegend, 2.5 µg/ml), anti-mouse Sca-1 antibody (APC-Cy7-conjugated, clone D7; Biolegend, 2.5 µg/ml), anti-mouse EpCAM antibody (eF450-conjugated, clone G8.8; eBioscience, 10 µg/ml) and anti-mouse PDGFRα antibody (APC-conjugated, clone APA5; Biolegend, 10 µg/ml) or corresponding isotype antibodies for 20 min at 4°C. Results were visualized by the FlowJo package (Tree Star Inc., Ashland, OR, USA).

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Manufacturer protocol

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