PE Rat Anti-Mouse CD73

Flow cytometry Anti-bodies Mouse - CD73

Experiment
Flow cytometry Anti-bodies Mouse - CD73
Product
PE Rat Anti-Mouse CD73 from BD Biosciences
Manufacturer
BD Biosciences

Protocol tips

Protocol tips
Each cell type was single cell suspended and, incubated in FACS buffer containing pre-conjugated antibodies (see supplementary Table 2) for 20 min on ice. Following incubation, cells were washed twice with FACS buffer.
Downstream tips
All the flow cytometry performed using a Cell Lab Quanta SCMPL. The data were analyzed using Kaluza®1.2 software (Beckman Coulter, Denmark) and the expression of each CD markers on the cells was calculated based on the percentage and mean fluorescence intensity (MFI).

Publication protocol

Cells were profiled for surface markers to identify their immune-phenotype. Each cell type was single cell suspended and, incubated in FACS buffer containing pre-conjugated antibodies (see supplementary Table 2) for 20 min on ice. Following incubation, cells were washed twice with FACS buffer. All the flow cytometry performed using a Cell Lab Quanta SCMPL. The data were analyzed using Kaluza®1.2 software (Beckman Coulter, Denmark) and the expression of each CD markers on the cells was calculated based on the percentage and mean fluorescence intensity (MFI).

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Manufacturer protocol

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