FITC anti-human/mouse Granzyme B Antibody

Protocol tips

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	IgG isotype controls were used as parallels. At last, we applied ALSRFortessa flow cytometry platform and Flowjo Software to analyze output data.

Protocol tips
IgG isotype controls were used as parallels. At last, we applied ALSRFortessa flow cytometry platform and Flowjo Software to analyze output data.

Publication protocol

Hepatic monocular lymphocytes were isolated as described [28]. For flowcytometry, after incubation with Fc-receptor blockage for 15 min at 4 °C, NK1.1, CD3, CD69, CD27, CD11b, CD49a, DX5, Ly49D, Ly49G2, Ly49H, Ly49I, NKG2A, NKG2D, granzyme B, IFN-γ, TNF-α molecules were marked with relevant antibodies according manufactory protocols (Additional file 1: Table S1) in order to analysis of some inhibitory/activating molecules and cytokines dynamics on NK cells. Moreover, as for intracellular cytokine marking, approximately 1 × 10^6 monocular lymphocytes were stimulated using Cell Stimulation Cocktail for 4 h [28]. IgG isotype controls were used as parallels. At last, we applied ALSRFortessa flow cytometry platform and Flowjo Software to analyze output data.

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