CD133 (Prominin-1) Monoclonal Antibody (13A4), APC, eBioscience™

Protocol tips

Upstream tips	Protocol tips	Downstream tips
	cells were incubated on ice for 30min with appropriate antibodies or isotype controls washed twice with FACS buffer (PBS containing 0.1% NaN3 and 5% fetal bovine serum), and resuspended in 0.5 ml 1% formalin/PBS.	All analyses were performed on a FACS calibur system (Becton Dickinson Immunocytometry Systems).

Protocol tips
cells were incubated on ice for 30min with appropriate antibodies or isotype controls washed twice with FACS buffer (PBS containing 0.1% NaN3 and 5% fetal bovine serum), and resuspended in 0.5 ml 1% formalin/PBS.
Downstream tips
All analyses were performed on a FACS calibur system (Becton Dickinson Immunocytometry Systems).

Publication protocol

To detect surface B7-H4, CD11b or CD133 expression, cells were incubated on ice for 30min with appropriate antibodies or isotype controls (PE-B7-H4, PE-mouse IgG1, APC-CD11b, APC-CD133, APC-mouse IgG1, all from eBioscience, 1:5), washed twice with FACS buffer (PBS containing 0.1% NaN3 and 5% fetal bovine serum), and resuspended in 0.5 ml 1% formalin/PBS. To analyze intracellular B7-H4, cells were pre-treated with Fix and Perm cell permeabilization reagents (Caltag Laboratories) according to the manufacturer's instructions. Assays of immune function, cell proliferation, cell cycle, apoptosis, cytokines and cytotoxicity were done under standard methods as previously described. All analyses were performed on a FACS calibur system (Becton Dickinson Immunocytometry Systems).

Full paper   Login or join for free to view the full paper.

Reviews

Discussion

Papers

Check out relevant papers found by Labettor's AI that are relevant for performing Flow cytometry Anti-bodies Human - CD133 using CD133 (Prominin-1) Monoclonal Antibody (13A4), APC, eBioscience™ from eBioscience.

Manufacturer protocol

Download the product protocol from eBioscience for CD133 (Prominin-1) Monoclonal Antibody (13A4), APC, eBioscience™ below.

Download manufacturer protocol

Videos

Check out videos that might be relevant for performing Flow cytometry Anti-bodies Human - CD133 using CD133 (Prominin-1) Monoclonal Antibody (13A4), APC, eBioscience™ from eBioscience. Please note that these videos are representative and steps or experiment specific processes must be kept in mind to expect desired results.