BV510 Mouse Anti-Human CD38

Protocol tips

Upstream tips	Protocol tips	Downstream tips
	cells were transferred, centrifugation at 1500 g for 10 min and incubated with antibodies for 20 min at 37 °C. Annexin V was then added and stained for 15 min at room temperature. After incubation, BMMCs were washed, centrifuged, measured by a FACSCanto II (BD Biosciences) and resulting data were analysed using FlowJo software.

Protocol tips
cells were transferred, centrifugation at 1500 g for 10 min and incubated with antibodies for 20 min at 37 °C. Annexin V was then added and stained for 15 min at room temperature. After incubation, BMMCs were washed, centrifuged, measured by a FACSCanto II (BD Biosciences) and resulting data were analysed using FlowJo software.

Publication protocol

Using the EasySep magnet to exclude infiltrating cells, adipocytes were stained with antibodies against anti-CD31 to identify endothelial cells, anti-CD105 for mesenchymal cells, anti-CD24 for stroma cells, LipidTox for neutral lipid droplets, and ant-CD36 for the differentiation stage of adipocytes (Fig. S3a). To analyse the cell survival of isolated BMMCs, cells were transferred, centrifugation at 1500 g for 10 min and incubated with antibodies against anti-human CD27 (BD Biosciences Cat#562513, RRID:AB_11153497), CD38 (BD Biosciences Cat# 563251, RRID:AB_2738097), Annexin V (BD Biosciences Cat# 556419, RRID:AB_2665412), anti-human CD3 (BioLegend Cat# 300407, RRID:AB_314061), anti-CD14 (BioLegend Cat# 325618, RRID:AB_830691), anti-human CD138 (Miltenyi Biotec Cat# 130-117-544, RRID:AB_2751398), CD19 (BD Biosciences Cat# 557791, RRID:AB_396873), and 7AAD (Milteny Biotec) for 20 min at 37 °C. Annexin V was then added and stained for 15 min at room temperature. After incubation, BMMCs were washed, centrifuged, measured by a FACSCanto II (BD Biosciences) and resulting data were analysed using FlowJo software.

Full paper   Login or join for free to view the full paper.

Reviews

Discussion

Papers

Check out relevant papers found by Labettor's AI that are relevant for performing Flow cytometry Anti-bodies Human - CD38 using BV510 Mouse Anti-Human CD38 from BD Biosciences.

Manufacturer protocol

Download the product protocol from BD Biosciences for BV510 Mouse Anti-Human CD38 below.

Download manufacturer protocol

Videos

Check out videos that might be relevant for performing Flow cytometry Anti-bodies Human - CD38 using BV510 Mouse Anti-Human CD38 from BD Biosciences. Please note that these videos are representative and steps or experiment specific processes must be kept in mind to expect desired results.