MboII restriction enzyme

Restriction Enzymes MboII

Experiment

Restriction Enzymes MboII

Product

MboII restriction enzyme from Takara Bio Inc

Manufacturer

Takara Bio Inc

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Protocol tips

Upstream tips	Protocol tips	Downstream tips
	Each DNA was incubated with 10 U restriction enzyme [MboII (Takara), AlwI (New England Biolabs, Beverly, USA) or MboI (Takara)] in 10 μl of a buffer {MboII buffer [10 mM Tris–HCl (pH 7.5), 10 mM MgCl2 and 1 mM DTT], AlwI buffer [20 mM Tris–HCl (pH 7.9), 10 mM MgCl2, 1 mM DTT and 50 mM KCl] and MboI buffer [20 mM Tris–HCl (pH 8.5), 10 mM MgCl2, 1 mM DTT and 100 mM KCl]} at 37°C for 1 h. The surrounding sequences of the recognition sequences are shown in Figure 1

Protocol tips
Each DNA was incubated with 10 U restriction enzyme [MboII (Takara), AlwI (New England Biolabs, Beverly, USA) or MboI (Takara)] in 10 μl of a buffer {MboII buffer [10 mM Tris–HCl (pH 7.5), 10 mM MgCl2 and 1 mM DTT], AlwI buffer [20 mM Tris–HCl (pH 7.9), 10 mM MgCl2, 1 mM DTT and 50 mM KCl] and MboI buffer [20 mM Tris–HCl (pH 8.5), 10 mM MgCl2, 1 mM DTT and 100 mM KCl]} at 37°C for 1 h. The surrounding sequences of the recognition sequences are shown in Figure 1

Protocol tips

Each DNA was incubated with 10 U restriction enzyme [MboII (Takara), AlwI (New England Biolabs, Beverly, USA) or MboI (Takara)] in 10 μl of a buffer {MboII buffer [10 mM Tris–HCl (pH 7.5), 10 mM MgCl2 and 1 mM DTT], AlwI buffer [20 mM Tris–HCl (pH 7.9), 10 mM MgCl2, 1 mM DTT and 50 mM KCl] and MboI buffer [20 mM Tris–HCl (pH 8.5), 10 mM MgCl2, 1 mM DTT and 100 mM KCl]} at 37°C for 1 h. The surrounding sequences of the recognition sequences are shown in Figure 1

Publication protocol

The pre-5′-half and the pre-3′-half PCR products were prepared using the ligation products recovered by ethanol precipitation. Each DNA was incubated with 10 U restriction enzyme [MboII (Takara), AlwI (New England Biolabs, Beverly, USA) or MboI (Takara)] in 10 μl of a buffer {MboII buffer [10 mM Tris–HCl (pH 7.5), 10 mM MgCl2 and 1 mM DTT], AlwI buffer [20 mM Tris–HCl (pH 7.9), 10 mM MgCl2, 1 mM DTT and 50 mM KCl] and MboI buffer [20 mM Tris–HCl (pH 8.5), 10 mM MgCl2, 1 mM DTT and 100 mM KCl]} at 37°C for 1 h. The surrounding sequences of the recognition sequences are shown in Figure 1

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Manufacturer protocol

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