MseI (RspRSII) restriction enzyme

Protocol tips

Upstream tips	Protocol tips	Downstream tips
	PCR products were digested with the MseI (RspRSII) restriction enzymes (Takara) in a total volume of 20 μl at 60°C for 1 h based on the manufacturer’s instructions, with some modifications.	The digested fragments were separated in 2% agarose gels by electrophoresis in TBE buffer for approximately 45 min and visualized by staining with ethidium bromide.

Protocol tips
PCR products were digested with the MseI (RspRSII) restriction enzymes (Takara) in a total volume of 20 μl at 60°C for 1 h based on the manufacturer’s instructions, with some modifications.
Downstream tips
The digested fragments were separated in 2% agarose gels by electrophoresis in TBE buffer for approximately 45 min and visualized by staining with ethidium bromide.

Publication protocol

Based on the DNA sequence information obtained in this study, we surveyed the restriction sites of the SBE locus extensively using Geneious Pro 7.1.5 (Biomatters Ltd.). The restriction enzymes with digestion sites that were conserved within a species and variable among other species in a given sequence were selected. The intron 11 of the SBE gene was used for the identification of A. cruentus. A fragment from the SBE gene of 278 bp (position 3,536 to 3,240) containing an MseI restriction site was amplified by PCR using the primers crsbe-F: 5′-AGCGAATTGCGACGAATTATGTTACAT-3′ and crsbe-R: 5′-TTCCTTTTCCACCGAACATCAATGCAT-3′. PCR conditions were as follows: 30 cycles of 98°C for 10 s, 55°C for 30 s and 72°C for 30 s. PCR products were digested with the MseI (RspRSII) restriction enzymes (Takara) in a total volume of 20 μl at 60°C for 1 h based on the manufacturer’s instructions, with some modifications. The digested fragments were separated in 2% agarose gels by electrophoresis in TBE buffer for approximately 45 min and visualized by staining with ethidium bromide.

Full paper   Login or join for free to view the full paper.

Reviews

Discussion

Papers

Check out relevant papers found by Labettor's AI that are relevant for performing Restriction Enzymes MseI / Tru1I using MseI (RspRSII) restriction enzyme from Takara Bio Inc.

Manufacturer protocol

Download the product protocol from Takara Bio Inc for MseI (RspRSII) restriction enzyme below.

Download manufacturer protocol

Videos

Check out videos that might be relevant for performing Restriction Enzymes MseI / Tru1I using MseI (RspRSII) restriction enzyme from Takara Bio Inc. Please note that these videos are representative and steps or experiment specific processes must be kept in mind to expect desired results.