FastDigest Tru1I

Restriction Enzymes MseI / Tru1I

Experiment
Restriction Enzymes MseI / Tru1I
Product
FastDigest Tru1I from Thermo Fisher Scientific
Manufacturer
Thermo Fisher Scientific

Protocol tips

Protocol tips
The nested PCR product was digested with TaaI and Tru1I FastDigest enzymes (Thermo Fisher Scientific Inc., Waltham, MA, USA) that produce diagnostic band patterns in agarose gel allowing to identify the two targeted species. With TaaI enzyme, brown trout DNA gives two bands of 205 and 272 bp, and with Tru1I, rainbow trout DNA gives a band of 66 bp.

Publication protocol

"Species-specific PCR-RFLP
The nested PCR-RFLP method was developed as described in Clusa et al. (2017) for
detecting the two targeted species (brown and rainbow trout). Briefly: a first PCR was
carried out to amplify a 567 bp fragment of the 16S rRNA gene, using as forward the 16Snew-F primer designed in the cited research (Table 3), and the reverse 16S-Br universal
primer from Palumbi et al. (2002). A nested PCR amplification was then performed with
the pair of Salmonidae-specific primers described in Zaiko et al. (2015) (Table 3). The
nested PCR product was digested with TaaI and Tru1I FastDigest enzymes (Thermo Fisher
Scientific Inc., Waltham, MA, USA) that produce diagnostic band patterns in agarose gel
allowing to identify the two targeted species. With TaaI enzyme, brown trout DNA gives
two bands of 205 and 272 bp, and with Tru1I, rainbow trout DNA gives a band of 66 bp"

Full paper   Login or join for free to view the full paper.

Reviews

FastDigest Tru1I from Thermo Fisher Scientific has not yet been reviewed for this experiment

We'd love it if you would be the first to write a review!

Discussion

Start your discussion

Share your thoughts or question with experts in your field

Start a discussion

Papers

Check out relevant papers found by Labettor's AI that are relevant for performing Restriction Enzymes MseI / Tru1I using FastDigest Tru1I from Thermo Fisher Scientific.

View full paper   Login or join for free to view the full paper.

Manufacturer protocol

Download the product protocol from Thermo Fisher Scientific for FastDigest Tru1I below.

Download PDF Download manufacturer protocol

Videos

Check out videos that might be relevant for performing Restriction Enzymes MseI / Tru1I using FastDigest Tru1I from Thermo Fisher Scientific. Please note that these videos are representative and steps or experiment specific processes must be kept in mind to expect desired results.

We haven't found any additional videos for this experiment / product combination yet.

Outsource your experiment

Fill out your contact details and receive price quotes in your Inbox

  Outsource experiment
Become shareholder Discussions About us Contact Privacy Terms