BstPI (BstEII, EcoO65I) restriction enzyme

Protocol tips

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	The restriction endonucleases BamHI, BstPI, DraI, EcoRI, Sau3AI, and SmaI (Takara Shuzo Co.); DpnI, HincII, and HindIII (New England BioLabs); and EcoRV (Nippon Gene) were used. Phage T4 DNA ligase, the Klenow fragment of E. coli DNA polymerase I, alkaline phosphatase (E. coli C75) (Takara Shuzo Co.), and Si nuclease (Sankyo Co.) were also used. The reaction conditions for these enzymes were as recommended by the suppliers.

Protocol tips

The restriction endonucleases BamHI, BstPI, DraI, EcoRI, Sau3AI, and SmaI (Takara Shuzo Co.); DpnI, HincII, and HindIII (New England BioLabs); and EcoRV (Nippon Gene) were used. Phage T4 DNA ligase, the Klenow fragment of E. coli DNA polymerase I, alkaline phosphatase (E. coli C75) (Takara Shuzo Co.), and Si nuclease (Sankyo Co.) were also used. The reaction conditions for these enzymes were as recommended by the suppliers.

Publication protocol

The restriction endonucleases BamHI, BstPI, DraI, EcoRI, Sau3AI, and SmaI (Takara Shuzo Co.); DpnI, HincII, and HindIII (New England BioLabs); and EcoRV (Nippon Gene) were used. Phage T4 DNA ligase, the Klenow fragment of E. coli DNA polymerase I, alkaline phosphatase (E. coli C75) (Takara Shuzo Co.), and Si nuclease (Sankyo Co.) were also used. The reaction conditions for these enzymes were as recommended by the suppliers

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Manufacturer protocol

Download the product protocol from Takara Bio Inc for BstPI (BstEII, EcoO65I) restriction enzyme below.

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