Bsp68I (NruI) (10 U/µL)

Restriction Enzymes NruI / Bsp68I

Experiment
Restriction Enzymes NruI / Bsp68I
Product
Bsp68I (NruI) (10 U/µL) from Thermo Fisher Scientific
Manufacturer
Thermo Fisher Scientific

Protocol tips

Protocol tips
Restriction digestion of the 300-bp product, obtained from PCR of all the clinical isolates, the reference strains, and the spiked sputum sample, was carried out with 1 U each of NruI and BamHI (Fermentas Life Sciences, Lithuania) in separate tubes. Restriction digestion was carried out with 10 μl of the amplicon at 37°C for 2 h.
Downstream tips
The products were electrophoresed on 2.5% agarose gel with a 50-bp DNA marker (Fermentas Life Sciences, Lithuania).

Publication protocol

Restriction digestion of the 300-bp product, obtained from PCR of all the clinical isolates, the reference strains, and the spiked sputum sample, was carried out with 1 U each of NruI and BamHI (Fermentas Life Sciences, Lithuania) in separate tubes. Restriction digestion was carried out with 10 μl of the amplicon at 37°C for 2 h. The products were electrophoresed on 2.5% agarose gel with a 50-bp DNA marker (Fermentas Life Sciences, Lithuania).

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Papers

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Manufacturer protocol

Download the product protocol from Thermo Fisher Scientific for Bsp68I (NruI) (10 U/µL) below.

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