VpaK11BI (AvaII) restriction enzyme

Restriction Enzymes AvaII / Eco47I / VpaK11BI

Experiment
Restriction Enzymes AvaII / Eco47I / VpaK11BI
Product
VpaK11BI (AvaII) restriction enzyme from Takara Bio Inc
Manufacturer
Takara Bio Inc

Protocol tips

Protocol tips
After completing duplex PCR, PRA using 2 restriction enzymes, AvaII (TaKaRa) and HaeIII (TaKaRa), was performed to identify the 105 NTM strains that produced 515-bp hsp65 DNA. PRA reactions were performed as follows. Ten microliters of PCR products was transferred to a fresh microcentrifuge tube and digested with restriction enzymes according to the manufacturer's instructions. Following digestion, mixtures were electrophoresed on 3% agarose gel gels.

Publication protocol

A PRA algorithm for differentiating mycobacteria was constructed using MapDraw (version 3.14; DNASTAR, Madison, Wis.). After completing duplex PCR, PRA using 2 restriction enzymes, AvaII (TaKaRa) and HaeIII (TaKaRa), was performed to identify the 105 NTM strains that produced 515-bp hsp65 DNA. PRA reactions were performed as follows. Ten microliters of PCR products was transferred to a fresh microcentrifuge tube and digested with restriction enzymes according to the manufacturer’s instructions. Following digestion, mixtures were electrophoresed on 3% agarose gel gels.

Full paper   Login or join for free to view the full paper.

Reviews

VpaK11BI (AvaII) restriction enzyme from Takara Bio Inc has not yet been reviewed for this experiment

We'd love it if you would be the first to write a review!

Discussion

Start your discussion

Share your thoughts or question with experts in your field

Start a discussion

Papers

Check out relevant papers found by Labettor's AI that are relevant for performing Restriction Enzymes AvaII / Eco47I / VpaK11BI using VpaK11BI (AvaII) restriction enzyme from Takara Bio Inc.

View full paper   Login or join for free to view the full paper.

Manufacturer protocol

Download the product protocol from Takara Bio Inc for VpaK11BI (AvaII) restriction enzyme below.

Download PDF Download manufacturer protocol

Videos

Check out videos that might be relevant for performing Restriction Enzymes AvaII / Eco47I / VpaK11BI using VpaK11BI (AvaII) restriction enzyme from Takara Bio Inc. Please note that these videos are representative and steps or experiment specific processes must be kept in mind to expect desired results.

We haven't found any additional videos for this experiment / product combination yet.

Outsource your experiment

Fill out your contact details and receive price quotes in your Inbox

  Outsource experiment
Become shareholder Discussions About us Contact Privacy Terms