FastDigest ApaI

Restriction Enzymes ApaI

Experiment
Restriction Enzymes ApaI
Product
FastDigest ApaI from Thermo Fisher Scientific
Manufacturer
Thermo Fisher Scientific

Protocol tips

Protocol tips
2200 bp bands amplified from gDNA of five +B and five 0B mice were removed from the agarose gels, purified with QIAquick Gel Extraction Kit (Qiagen) and then digested with twelve restriction endonucleases (RE): ApaI, HindIII, Bsp120I, MfeI, HpaI, MspI, MboI, AluI, MseI, Ecl136II, HaeIII and SacI (FastDigest, Thermo Scientific, Waltham, MA USA) according to the manufacturer’s instructions. Digested DNA fragments were separated by agarose gel electrophoresis, visualized and photo-documented as above.

Publication protocol

"Restriction Fragment Length Polymorphism (RFLP) Analysis
2200 bp bands amplified from gDNA of five +B and five 0B mice were removed from the agarose gels, purified with QIAquick Gel Extraction Kit (Qiagen) and then digested with twelve restriction endonucleases (RE): ApaI, HindIII, Bsp120I, MfeI, HpaI, MspI, MboI, AluI, MseI, Ecl136II, HaeIII and SacI (FastDigest, Thermo Scientific, Waltham, MA USA) according to the manufacturer’s instructions. Digested DNA fragments were separated by agarose gel electrophoresis, visualized and photo-documented as above."

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Manufacturer protocol

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