NaeI NEB#R0190

Protocol tips

Upstream tips	Protocol tips	Downstream tips
	For the NaeI experiments, 50 units ml−1 NaeI (NEB), were added in NEBuffer 2 (NEB) supplemented with 100 μg ml−1 BSA and 3.66 μg ml−1 unlabeled background dsDNA.

Protocol tips
For the NaeI experiments, 50 units ml−1 NaeI (NEB), were added in NEBuffer 2 (NEB) supplemented with 100 μg ml−1 BSA and 3.66 μg ml−1 unlabeled background dsDNA.

Publication protocol

The basic magnetic tweezers protocol has been described (19). Images were acquired with a frequency of 60 Hz. In brief, DNA constructs were bound to 1-μm streptavidin-coated superparamagnetic microspheres (Invitrogen) and flushed into a flow cell, with a bottom cover slip coated with anti-digoxigenin. Subsequently, 15 nM EcoPI or EcoP15I were added in buffer R (50 mM Tris·HCl, pH 8.0, 50 mM KCl, 10 mM MgCl2, 1 mM DTT, and 100 μg ml−1 BSA, 20 ± 2°C) plus 4 mM ATP. Where indicated the buffer was supplemented with 100 μM AdoMet. For the NaeI experiments, 50 units ml−1 NaeI (NEB), were added in NEBuffer 2 (NEB) supplemented with 100 μg ml−1 BSA and 3.66 μg ml−1 unlabeled background dsDNA.

Full paper   Login or join for free to view the full paper.

Reviews

Discussion

Papers

Check out relevant papers found by Labettor's AI that are relevant for performing Restriction Enzymes PdiI / NaeI using NaeI NEB#R0190 from New England BioLabs.

Manufacturer protocol

Download the product protocol from New England BioLabs for NaeI NEB#R0190 below.

Videos

Check out videos that might be relevant for performing Restriction Enzymes PdiI / NaeI using NaeI NEB#R0190 from New England BioLabs. Please note that these videos are representative and steps or experiment specific processes must be kept in mind to expect desired results.