Eco52I (XmaIII) restriction enzyme

Protocol tips

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	To further confirm LAMP assay results, the amplified products were digested with restriction enzymes and the fragment sizes analyzed by electrophoresis (Eco52I for Beijing genotype strain amplicons) (Fig. 2). Briefly, one microliter of LAMP products was digested overnight by 10 U of Eco52I (Takara, Shiga, Japan) at 37°C and the digested products analyzed by 2% agarose gel electrophoresis.

Protocol tips
To further confirm LAMP assay results, the amplified products were digested with restriction enzymes and the fragment sizes analyzed by electrophoresis (Eco52I for Beijing genotype strain amplicons) (Fig. 2). Briefly, one microliter of LAMP products was digested overnight by 10 U of Eco52I (Takara, Shiga, Japan) at 37°C and the digested products analyzed by 2% agarose gel electrophoresis.

Publication protocol

Some of the amplified products were detected by agarose gel electrophoresis to assess the sensitivity of LAMP assay. To further confirm LAMP assay results, the amplified products were digested with restriction enzymes and the fragment sizes analyzed by electrophoresis (Eco52I for Beijing genotype strain amplicons) (Fig. 2). Briefly, one microliter of LAMP products was digested overnight by 10 U of Eco52I (Takara, Shiga, Japan) at 37°C and the digested products analyzed by 2% agarose gel electrophoresis.

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