BfmI (SfcI) (10 U/µL)

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	TERT rs2736100 was digested with restriction enzyme SfcI (BfmI) (Thermo Fisher Scientific Inc.).

Protocol tips
TERT rs2736100 was digested with restriction enzyme SfcI (BfmI) (Thermo Fisher Scientific Inc.).

Publication protocol

"All three SNPs (rs402710C/T in the CLPTM1L gene;
rs2736100A/C and rs2736098 G/A in the TERT gene)
were genotyped using the polymerase chain reaction
(PCR), restriction fragment length polymorphism
(RFLP) method, as described previously (11,12). Primers
and product lengths are given in Table 1.
After digestion by Pvu II (Thermo Fisher Scientific
Inc., Waltham, MA, USA), the CLPTM1L rs402710
product with the C allele remained as one fragment of
473 bp, and the T allele product was divided into fragments of 263 and 210 bp. TERT rs2736100 was digested
with restriction enzyme SfcI (BfmI) (Thermo Fisher
Scientific Inc.). The A allele produced a single 152-bp
fragment and the C allele produced two fragments of 104
and 48 bp. TERT rs2736098 was digested with Bsp120I
(Thermo Fisher Scientific Inc.). Two amplification
products (289 bp and 90 bp) were generated from the G allele, whereas the G>A polymorphism generated only
one 379-bp fragment.
All digestion products were loaded on 8% polyacrylamide gel, stained with ethidium bromide, and observed
on an ultraviolet fluorescence imaging system. "

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Manufacturer protocol

Download the product protocol from Thermo Fisher Scientific for BfmI (SfcI) (10 U/µL) below.

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