PdmI (XmnI) (10 U/µL)

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	We digested the PCR products with PdmI (XmnI) (Thermo Scientific).

Protocol tips
We digested the PCR products with PdmI (XmnI) (Thermo Scientific).

Publication protocol

We extracted DNA from plants using the MasterPure Complete DNA Purification Kit (Epicentre, Madison, WI). To genotype plants for ASY1 diploid (D) versus tetraploid (T) alleles, we first amplified an ∼300-bp fragment with primers F1 (5′-TTT​GGT​TTT​CGT​TTT​GCT​GA-3′) and R1 (5′- GAG​ATT​CAG​CGT​CCA​TAG​GC-3′). We digested the PCR products with PdmI (XmnI) (Thermo Scientific). The diploid allele gives digestion products of ∼200 and ∼100 bp. The tetraploid allele remains undigested. To genotype for ASY3 D vs. T alleles, we amplified an ∼300-bp fragment of the ASY3 gene with primers F1 (5′-GCC​CTG​AAA​ATG​CTA​CCA​GAA​GGC​CAG​TGA-3′) and R1 (5′-GCG​AAA​ATG​AGC​CTT​ACG​AC-3′). We digested the PCR product with NmuCI (Tsp45I) restriction enzyme (Thermo Scientific). The diploid allele gives digestion products of ∼200 and ∼100 bp while the tetraploid allele remains undigested.

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Manufacturer protocol

Download the product protocol from Thermo Fisher Scientific for PdmI (XmnI) (10 U/µL) below.

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