TasI (Tsp509I) (10 U/µL)

Protocol tips

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	Tsp509I fast digest restriction enzyme (Fermentas®) was selected for RFLP method that caused the separation specifically of Fasciola species. To performance RFLP, 3 µl of Fasciola ITS1, 5.8S, ITS2 PCR product, 1 µl of supplied restriction enzyme buffer, 0.5 µl of restriction enzyme, and 10.5 µl DDW in total volume 15 µl were incubated at 65°C for 1 h. Digestion products were analyzed and photographed on 2.5% agarose gel.

Protocol tips

Tsp509I fast digest restriction enzyme (Fermentas®) was selected for RFLP method that caused the separation specifically of Fasciola species. To performance RFLP, 3 µl of Fasciola ITS1, 5.8S, ITS2 PCR product, 1 µl of supplied restriction enzyme buffer, 0.5 µl of restriction enzyme, and 10.5 µl DDW in total volume 15 µl were incubated at 65°C for 1 h. Digestion products were analyzed and photographed on 2.5% agarose gel.

Publication protocol

Tsp509I fast digest restriction enzyme (Fermentas®) was selected for RFLP method that caused the separation specifically of Fasciola species. To performance RFLP, 3 µl of Fasciola ITS1, 5.8S, ITS2 PCR product, 1 µl of supplied restriction enzyme buffer, 0.5 µl of restriction enzyme, and 10.5 µl DDW in total volume 15 µl were incubated at 65°C for 1 h. Digestion products were analyzed and photographed on 2.5% agarose gel.

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Download the product protocol from Thermo Fisher Scientific for TasI (Tsp509I) (10 U/µL) below.

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