Hin1II (NlaIII) (5 U/µL)

Protocol tips

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	Following DNA amplification, double digestion of PCR products was performed according to the manufacturer's instructions using the AluI enzyme recognising AG∧CT sites (Thermo Fisher Scientific, Waltham, Massachusetts, USA, catalogue no. ER0011) and the Hin1II (NlaIII) enzyme recognising CATG∧ sites (Thermo Fisher Scientific, catalogue no. ER1831). Briefly, 10 μl of the PCR reaction mixture was mixed with 18 μl of Molecular Biology Reagent Water (Sigma-Aldrich, Saint Louis, Missouri, USA), 2 μl of 10X Buffer Tango, 2 μl of AluI and 4 μl of Hin1II. The mixture was spun down for 30 s and incubated at 37 °C overnight.

Protocol tips

Following DNA amplification, double digestion of PCR products was performed according to the manufacturer's instructions using the AluI enzyme recognising AG∧CT sites (Thermo Fisher Scientific, Waltham, Massachusetts, USA, catalogue no. ER0011) and the Hin1II (NlaIII) enzyme recognising CATG∧ sites (Thermo Fisher Scientific, catalogue no. ER1831). Briefly, 10 μl of the PCR reaction mixture was mixed with 18 μl of Molecular Biology Reagent Water (Sigma-Aldrich, Saint Louis, Missouri, USA), 2 μl of 10X Buffer Tango, 2 μl of AluI and 4 μl of Hin1II. The mixture was spun down for 30 s and incubated at 37 °C overnight.

Publication protocol

Following DNA amplification, double digestion of PCR products was performed according to the manufacturer's instructions using the AluI enzyme recognising AG∧CT sites (Thermo Fisher Scientific, Waltham, Massachusetts, USA, catalogue no. ER0011) and the Hin1II (NlaIII) enzyme recognising CATG∧ sites (Thermo Fisher Scientific, catalogue no. ER1831). Briefly, 10 μl of the PCR reaction mixture was mixed with 18 μl of Molecular Biology Reagent Water (Sigma-Aldrich, Saint Louis, Missouri, USA), 2 μl of 10X Buffer Tango, 2 μl of AluI and 4 μl of Hin1II. The mixture was spun down for 30 s and incubated at 37 °C overnight.

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Manufacturer protocol

Download the product protocol from Thermo Fisher Scientific for Hin1II (NlaIII) (5 U/µL) below.

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