BrdU antibody

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Publication protocol

All sections were treated equally using a standard free-floating protocol. For BrdU detection, sections were pretreated in 2 N HCl (30 min, 37°C) and neutralized in 0.1 M Tris-buffered saline (pH 8.5, 10 min). Preincubation with 0.25% Triton X-100 and 10% normal serum for 30 min was followed by incubation with the primary antibody (4 h, RT + overnight, 4°C). The following primary antibodies were applied in consecutive sections to avoid overlap in quantification for either antibody: Goat polyclonal anti-DCX (1:500, sc8066, Santa Cruz Biotechnology Inc., Dallas, TX, USA), mouse monoclonal anti-Ki-67 (1:100, NCL-L-Ki67-MM1, Leica Biosystems Newcastle Ltd., Newcastle Upon Tyne, UK) or rabbit polyclonal anti-Ki-67 (1:500, ab15580, Abcam, Cambridge, UK) and rat monoclonal anti-BrdU (1:500, OBT0030, AbD Serotec, Oxford, UK).

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