pBad-RFP

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	For RFP expression, freshly transformed DH10B colonies were inoculated in triplicate into 5 mL LB medium and grown at 37°C and 180 rpm for 18 hours. 1 µL of each culture was then added to 150 µL of medium containing the appropriate concentration of inducer (either IPTG or arabinose) in a 96-well plate, and the plate was incubated at 37°C and 180 rpm for 18 hours and then analyzed. For time course fluorescence experiments, 15 µL of overnight cultures was added to 135 µL of fresh media, and plates were incubated in a Biotek Synergy H4 plate reader at 37°C with medium-strength shaking and analyzed every 20 minutes.

Protocol tips

For RFP expression, freshly transformed DH10B colonies were inoculated in triplicate into 5 mL LB medium and grown at 37°C and 180 rpm for 18 hours. 1 µL of each culture was then added to 150 µL of medium containing the appropriate concentration of inducer (either IPTG or arabinose) in a 96-well plate, and the plate was incubated at 37°C and 180 rpm for 18 hours and then analyzed. For time course fluorescence experiments, 15 µL of overnight cultures was added to 135 µL of fresh media, and plates were incubated in a Biotek Synergy H4 plate reader at 37°C with medium-strength shaking and analyzed every 20 minutes.

Publication protocol

For RFP expression, freshly transformed DH10B colonies were inoculated in triplicate into 5 mL LB medium and grown at 37°C and 180 rpm for 18 hours. 1 µL of each culture was then added to 150 µL of medium containing the appropriate concentration of inducer (either IPTG or arabinose) in a 96-well plate, and the plate was incubated at 37°C and 180 rpm for 18 hours and then analyzed. For time course fluorescence experiments, 15 µL of overnight cultures was added to 135 µL of fresh media, and plates were incubated in a Biotek Synergy H4 plate reader at 37°C with medium-strength shaking and analyzed every 20 minutes.

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