GenePrint® 10 System

Protocol tips

Upstream tips	Protocol tips	Downstream tips
	Use of a gender-determining marker, Amelogenin Use of appropriate positive and negative controls

Protocol tips
Use of a gender-determining marker, Amelogenin Use of appropriate positive and negative controls
Downstream tips

Publication protocol

STR profiling was carried out following the ANSI/ATCC ASN-0002-2011 guidance, Authentication of Human Cell Lines: Standardization of STR Profiling. Briefly, a Promega GenePrint 10 Kit was used to polymerase chain (PCR) amplify eight short tandem repeat (STR) loci plus a gender-determining marker, Amelogenin. The PCR product was electrophoresed on an ABI Prism® 3730xl Genetic Analyzer using an Internal Lane Standard 600 (Promega). Data was analyzed using GeneMapper version 4.0 software (Applied Biosystems). Appropriate positive and negative controls were used.
All STR experiments have been performed in one laboratory (JHU) by one individual to exclude any possible inter-laboratory and/or inter-operator effects on reproducibility.

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Manufacturer protocol

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