AmpFLSTR™ Identifiler™ Direct PCR Amplification Kit

Protocol tips

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	Follow the manufacturer’s instructions

Protocol tips
Follow the manufacturer’s instructions

Publication protocol

Cell line authentication using short tandem repeats: 1 µl of extract was PCR amplified using the Identifiler Direct STR profiling kit (catalog # 4467831), according to manufacturer’s instructions. 1 µl of PCR product was combined with HiDi formamide and LIZ600 sizing markers, denatured at 95°C for 3 min, and analyzed on a SeqStudio Genetic Analyzer using conditions in the default ‘fragment analysis’ module supplied on the instrument. One capillary was run with the allelic ladder provided in the Identifiler Direct kit. Alleles present in each sample were determined using GeneMapper 5 software and the appropriate panels and bins.

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Manufacturer protocol

Download the product protocol from Thermo Fisher Scientific for AmpFLSTR™ Identifiler™ Direct PCR Amplification Kit below.

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