CometAssay Single Cell Gel Electrophoresis Assay

Protocol tips

Upstream tips	Protocol tips	Downstream tips
Seed 1–2 × 10^4 cells/ml	Procedure for alkaline comet assay

Upstream tips
Seed 1–2 × 10^4 cells/ml
Protocol tips
Procedure for alkaline comet assay

Publication protocol

Alkaline comet assays were performed on mouse MECs engineered with CRISPR/Cas9 (gPlant/gRosa26/gNumb/ or gBrca1) using the CometAssay kit (Trevigen, 4250-050-K) according to the manufacturer’s instructions. ~ 100-300 cells were analyzed in each experiment.

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Manufacturer protocol

Download the product protocol from Bio-Techne for CometAssay Single Cell Gel Electrophoresis Assay below.

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