Comet Assay Kits, 96-Well

Protocol tips

Upstream tips	Protocol tips	Downstream tips
Resuspend the cells at 1 x 10^5 cells/mL in ice-cold PBS (without Mg2+ and Ca2+).		Comet tail lengths can be quantified by NIH ImageJ software. Each data point can be calculated as an average of 100 cells,

Upstream tips
Resuspend the cells at 1 x 10^5 cells/mL in ice-cold PBS (without Mg2+ and Ca2+).
Downstream tips
Comet tail lengths can be quantified by NIH ImageJ software. Each data point can be calculated as an average of 100 cells,

Publication protocol

Alkaline comet assays (Cell Biolabs STA-355) were performed to measure total DNA lesions, including DNA bases, single-strand breaks (SSBs), and DSBs, were assessed using single-cell gel electrophoresis. Slides were stained with SYBR green and visualized by using a Leica DM5500 microscope. Comet tail lengths were quantified by NIH ImageJ software. Each data point was calculated as an average of 100 cells, and samples were performed in triplicate for each treatment group.

Full paper   Login or join for free to view the full paper.

Reviews

Discussion

Papers

Check out relevant papers found by Labettor's AI that are relevant for performing DNA Damage Assay MDA-MB-231 using Comet Assay Kits, 96-Well from Cell Biolabs.

Manufacturer protocol

Download the product protocol from Cell Biolabs for Comet Assay Kits, 96-Well below.

Download manufacturer protocol

Videos

Check out videos that might be relevant for performing DNA Damage Assay MDA-MB-231 using Comet Assay Kits, 96-Well from Cell Biolabs. Please note that these videos are representative and steps or experiment specific processes must be kept in mind to expect desired results.