CometAssay Single Cell Gel Electrophoresis Assay

DNA Damage Assay MIA PaCa-2

Experiment
DNA Damage Assay MIA PaCa-2
Product
CometAssay Single Cell Gel Electrophoresis Assay from Bio-Techne
Manufacturer
Bio-Techne

Protocol tips

Upstream tips
Seed 1–2 × 10^4 cells/ml

Publication protocol

A plate-based immunoassay was used to detect the concentration of 8-oxo-dG, a marker of oxidative DNA damage (HT 8-oxo-dG ELISA Kit, R&D Systems, Minneapolis, MN, USA), and The Comet Assay Single Cell Gel Electrophoresis Assay (CN 4250-050-K; R&D Systems, Minneapolis, MN, USA) was used to measure DNA strand breaks following manufacturers’ instructions.

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Papers

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Manufacturer protocol

Download the product protocol from Bio-Techne for CometAssay Single Cell Gel Electrophoresis Assay below.

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