Biotinylated Protein Ladder Detection Pack #7727

Protein Ladder Immunofluorescence

Experiment

Protein Ladder Immunofluorescence

Product

Biotinylated Protein Ladder Detection Pack #7727 from Cell Signaling Technology

Manufacturer

Cell Signaling Technology

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Tips Publication protocol Reviews Discussion Papers Manufacturer protocol Videos

Protocol tips

Upstream tips	Protocol tips	Downstream tips
• The molecular weight ladder is a mixture of purified proteins covalently coupled to biotin that resolve to 10 bands that have a size range of 9-200 kDa.	• Heat the ladder to 95-100ºC for 2 minutes. • To ensure uniform mobility, load an equal volume of 1X Reducing SDS Loading Buffer into any unused wells. • Perform Chemiluminescent detection according to manufacturer's instructions except, at the stage of secondary antibody incubation with the blot. • Concurrently add the anti-biotin HRP-linked antibody (provided) at a dilution of 1:1000.

Upstream tips
• The molecular weight ladder is a mixture of purified proteins covalently coupled to biotin that resolve to 10 bands that have a size range of 9-200 kDa.
Protocol tips
• Heat the ladder to 95-100ºC for 2 minutes. • To ensure uniform mobility, load an equal volume of 1X Reducing SDS Loading Buffer into any unused wells. • Perform Chemiluminescent detection according to manufacturer's instructions except, at the stage of secondary antibody incubation with the blot. • Concurrently add the anti-biotin HRP-linked antibody (provided) at a dilution of 1:1000.

Publication protocol

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Papers

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Manufacturer protocol

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Videos

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