Acridine Orange solution

Protocol tips

Upstream tips	Protocol tips	Downstream tips
• Five percent acridine orange was used to identify autophagous cells incubatedin the dark for 15 min. The dye induced the red glow of the autophagous bodies.

Upstream tips
• Five percent acridine orange was used to identify autophagous cells incubatedin the dark for 15 min. The dye induced the red glow of the autophagous bodies.

Publication protocol

For identification of apoptosis and necrosis, a solution of propidium iodide (Sigma,St. Louis, MO, USA) and Hoechst 33342 (Sigma, St. Louis, MO, USA) in distilled water in a ratio of3:2:5 were used. The cells were stained upon incubation with the appropriate combinations of the drug.After addition of 2.5μL of the mixture to 1 mL of the medium, the tumor cells were incubated at 37◦Cfor 5 min. The morphological analysis was performed under a fluorescence microscope. It showed bright blue fluorescence characteristic of apoptotic cells. Necrotic cells emitted red-pink fluorescence. Five percent acridine orange was used to identify autophagous cells incubatedin the dark for 15 min.

Full paper   Login or join for free to view the full paper.

Reviews

Discussion

Papers

Check out relevant papers found by Labettor's AI that are relevant for performing Necrosis T98-G using Acridine Orange solution from Sigma-Aldrich.

Manufacturer protocol

Download the product protocol from Sigma-Aldrich for Acridine Orange solution below.

Videos

Check out videos that might be relevant for performing Necrosis T98-G using Acridine Orange solution from Sigma-Aldrich. Please note that these videos are representative and steps or experiment specific processes must be kept in mind to expect desired results.