jetPEI® DNA transfection, HTS application

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	Cells starved of glucose and/or glutamine for 24–36 hr were stimulated as indicated. Cells were transfected with JetPEI Polyplus reagent (Genycell Biotech) and treated as indicated 24 hr later.

Protocol tips
Cells starved of glucose and/or glutamine for 24–36 hr were stimulated as indicated. Cells were transfected with JetPEI Polyplus reagent (Genycell Biotech) and treated as indicated 24 hr later.

Publication protocol

Tumor enteroendocrine STC-1, ovary carcinoma OVCAR-3, mammary adenocarcinoma MDA-MB-231, and colorectal adenocarcinoma HT-29 cells were cultured in Dulbecco’s modified Eagle’s medium and pancreatic carcinoma AsPC-1 in RPMI. Both media were supplemented with 10% fetal bovine serum. Cells starved of glucose and/or glutamine for 24–36 hr were stimulated as indicated. Cells were transfected with JetPEI Polyplus reagent (Genycell Biotech) and treated as indicated 24 hr later. Recombinant Wnt-3a proteins were from PeproTech. The GloMax 96 Luminometer (Promega) and Dual-Luciferase kit were used. Fractionation was as in Andrews and Faller (1991).

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Manufacturer protocol

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